Virus Titering Assay Service
Isolating the virus from the cultured cells and then using experimental methods to qualitatively and quantitatively check the virus is the basis of virus research. Creative BioMart Vir-Sci offers quality virus titering assay services for customers that focus on viral therapeutics, virus researches, etc. Fast testing and preferential prices help customers shorten the research cycle and reduce research costs.
Virus Titering Assay
Virus titration is usually expressed as virus particles or infected particles per milliliter, depending on the type of assay. And the higher virus titration is usually related to the severity of active viral infection. The amount of virus per milliliter by experimental detection means can be calculated by estimating the amount of live virus particles in the sample solution involved. There are various inspection methods for virus tittering so far. Among them, the most commonly used methods in experiments and diagnosis are the virus plaque formation test and the determination of half the tissue culture infection dose TCID50.
Viral plaque detection is one of the most widely used viral titer detection methods. This method was first adopted to calculate the infectivity of phages, and since then, this method has been widely used for the quantification of various viruses. Usually, the virus particles in the monolayer of the initially infected cultured host cell will be surrounded by a small dot on the monolayer by uninfected cells (called plaque), so it is called plaque detection. It is worth noting that the plaque test is only applicable to viruses that can multiply and infect monolayer cultured cells and can damage the cells.
The plaque test is very useful for determining the virus titer. However, there are several types of viruses that do not cause cell death when cultured, thereby forming plaque. In this case, titers can be detected by TCID50, LD50, EID50 and other methods. Although these viruses do not cause cell death after infecting cells, they will denature after a certain time (5-20 days), which is a pathological effect (CPE). By determining the amount of virus that causes cytopathy in half of the monolayer cell culture, namely TCID50, the virus concentration obtained can be analyzed by statistical methods. Although the accuracy and reproducibility of virus titers obtained by this detection method are not very high, it is possible to obtain approximate titers and evaluate differences between treatment groups.
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Our Advantages
- Ensure high efficiency and quality for virus titering services
- Professional data processing and analysis
- Competitive price in the market of virus titering services
- Suitable for multiple virus titration detection
- Ensure 24/7 online service
- Short feedback cycle
Workflow of Virus Service at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of virus titering, involving various viruses and technical methods. We guarantee that each specific experimental step in the virus tittering assay is strictly controlled to ensure high quality virus service. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality virus titering solutions according to your needs and provide you with professional and thoughtful services.
References
- Lee, C.; et al. Chikungunya virus neutralization antigens and direct cell-to-cell transmission are revealed by human antibody-escape mutants. PLoS Pathog. 2011, 7: e1002390.
- LaBarre, D.D.; Lowy, R.J. Improvements in methods for calculating virus titer estimates from TCID50 and plaque assays. J Virol Methods. 2001, 96: 107-26.
- Forcic, D.; et al. Comparisons of mumps virus potency estimates obtained by 50% cell culture infective dose assay and plaque assay. Vaccine. 2010, 28: 1887-92.
- Roldão, A.; et al. Error assessment in recombinant baculovirus titration: evaluation of different methods. J Virol Methods. 2009, 159: 69-80.
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