Herpes Simplex Virus (HSV) Vector Service

Creative BioMart Vir-Sci is a world leader in virus engineering and packaging. With a deep understanding of the genetics and molecular biology of HSV, as well as our advanced facilities, scientists at Creative BioMart Vir-Sci have developed an excellent HSV vector production platform. We have many years of experience with HSV vectors and advanced production systems and equipment. We can provide fast and satisfactory HSV vector services according to customer needs.

Herpes Simplex Virus

Herpes simplex virus (HSV) is an enveloped, large linear and double-stranded DNA virus in the herpesviridae family. There are two types of HSV: herpes simplex virus type I (HSV-1) and herpes simplex virus type II (HSV-2), both of which can infect humans and cause disease. The HSV genome is replicated through a rolling circle mechanism, forming coenzymes from beginning to end. In HSV's large genomes, about half of the sequences are non-essential genes, which means they can be replaced by one or more transgenes.

Features

• HSV has a large insertion capacity.
• HSV can infect dividing and non-dividing cells.
• Continuous expression of long-term infection genes.
• The risk of insertional mutagenesis is small.
• Relatively stable, can be concentrated to high virus titer.
• Orientation of nerve cells for targeting neurons.

Herpes Simplex Virus Vector Construction

HSV is one of the most competitive drug candidates in gene delivery vectors. The HSV-1 vector is not integrated into the host genome, resulting in transient expression in infected cells. Most HSV vectors were developed from HSV-1 strains. HSV-1 infection can be bacteriolytic or latent. Partial deletion of the HSV gene results in expression vectors with low toxicity and excellent packaging capacity for foreign DNA greater than 30 kb. Generally, HSV-1 mutant strains that lack replication capacity to avoid a host immune response are used. The HSV-1 vector is designed to delete ICP0, ICP4, ICP22, ICP27 or ICP47 to reduce toxicity. Creative BioMart Vir-Sci uses advanced biomolecular technology and gene knockout technology to construct HSV vectors to meet the various needs of basic research and clinical trials. The design and construction of HSV vectors includes the knockout of multiple genes in the genome, which are characterized by the use of appropriate promoters and the insertion of foreign genes.

Advantages

• Big size.
• Wide host range.
• Build long-term asymptomatic infection ability in neuronal cells.
• Specific regions of the viral genome are still being expressed.

Our Service

• Target gene packaging design of the replication-defective HSV vector.
• Replication-deficient HSV vector construction services for target genes.
• Synthesize and subclone the target DNA into our HSV plasmid.
• Provide recombinant virus.

Workflow of Services at Creative BioMart Vir-Sci

Creative BioMart Vir-Sci has a team of experts with extensive experience in the field of HSV vectors, involving various viruses and technical methods. We guarantee strict control of each experimental step in the production process to ensure high quality and high yield of HSV vectors. Creative BioMart Vir-Sci provides global customers with the design and construction of target gene packaging for replication-defective HSV vectors. Please feel free to contact us for more detailed information, and our scientists will discuss your project further.

Reference

1. Kuypers, HG.; et al. Viruses as transneuronal tracers. Trends Neurosci. 1990;2: 71–5.