Plantago Asiatica Mosaic Virus (PlAMV) Vector System Service
Creative BioMart Vir-Scihas been committed to providing efficient and high-quality vector construction services for scientists in the field of botany. We have experts in both designing and engineering PIAMV based expression vectors. By using highly optimized and efficient protocols, we permit high quality vectors used for universal expression of exogenous proteins.
Plantago Asiatica Mosaic Virus (PlAMV) Based Expression System
Plantago asiatica mosaic virus (PlAMV), which belongs to the genus Potexvirus, is composed of a single-strand positive-sense RNA genome. A total of five open reading frames (ORFs) were encoded in PIAMV genome including RNA-dependent RNA polymerase (RdRp), three movement proteins named triple gene block (TGB) and a coat protein (CP). In the original attempt to repurpose PIAMV as a transient expression system in plants, a CP lacking the N-terminal amino acids was fused to the C-terminus of the GFP via the 2A peptide. Although GFP fluorescence was readily observed, this vector was not capable of infecting the plants systemically. The N-terminal coding region of CP is required for cell-to-cell movement of PIAMV, however, the native sequences greatly impaired the genetic stability of the resultant vectors. To this end, an optimized version was developed by introducing synonymous substitutions into the N-terminus of CP. In contrast to the previous version, the newly designed vector moved efficiently in the infected tissues of N. benthamiana.
Figure 1. Schematic illustration of plantago asiatica mosaic virus (PlAMV) based vectors. The gene of interest (GOI) is inserted between the coding region of TGB and CP. The self-cleaving 2A peptide sequence from the foot-and-mouth disease virus is used to link the GOI and CP. Several synonymous mutations are introduced to the N-terminus of CP coding region to avoid potential recombination events. LB: left border; RB: right border.
Features of Plantago Asiatica Mosaic Virus (PlAMV) Based Vectors
- High level of genetic stability due to optimized sequences of CP coding region.
- Preservation of all functional elements enables both cell-to-cell and systemic movement of the vector.
- Long term expression of foreign proteins due to robust activity of RNA silencing suppression.
- Wide range of host plant species including N. benthamiana and A. thaliana.
Service Advantages
- Smart design of PIAMV-based vector
- Highly standardized protocols
- Top quality vector with flexible production scale
- Strict QC testing to ensure correct sequences
- Competitive price in the market
Workflow of Construction of PIAMV Based Expression Vectors
The expert team in Creative BioMart Vir-Sci will always find the suitable solution to your research goal. Please feel free to contact us for further questions.
References
- Minato, N.; et al. Efficient foreign gene expression in planta using a plantago asiatica mosaic virus-based vector achieved by the strong RNA-silencing suppressor activity of TGBp1. Archives of virology. 2014, 159(5): 885-896.
- Ozeki, J.; et al. The N-terminal region of the Plantago asiatica mosaic virus coat protein is required for cell-to-cell movement but is dispensable for virion assembly. Molecular plant-microbe interactions. 2009, 22(6): 677-685.