Protein Expression and Purification Service
Creative BioMart Vir-Sci provides customers in academia and industry with protein expression and purification services based on the baculovirus/insect cell expression system, and uses the standard process recognized by the international academic community to satisfy all kinds of experiments. We have years of one-stop service experience in baculovirus/insect expression system, and our customized service solutions can meet the different needs of customers.
Protein Expression and Purification
Protein expression is related to the gene sequence and the corresponding mRNA sequence, both of them have a decisive influence on the protein expression. Gene sequence design and optimization can significantly improve the protein expression, and even make the insoluble protein become soluble.
Protein purification is the combination of a series of complex processes, including crude separation, preliminary purification and fine purification. Extraction, precipitation, centrifugation, chromatography and other means are optimized to yield high quality of protein product. Creative BioMart Vir-Sci has the top protein purification technicians, can design strategy according to the nature of the protein. Affinity purification, ion exchange, molecular sieve, hydrophobic and anti-equivalence of various protein purification experimental scheme are used to obtain high purity protein, which can meet the protein folding, recrystallization and other experimental requirements.
Figure 1. Schematic diagram of protein expression and purification for baculovirus/insect cell expression system.
Protein Expression and Purification Services
- Creative BioMart Vir-Sci is committed to providing customers with high purity and low cost for active recombinant protein product. With one-stop protein services from gene optimization synthesis, protein expression, purification and quality control.
- Creative BioMart Vir-Sci has advanced protein expression technology and rich experience in protein expression, and has completed the preparation of a variety of proteins that are not expressed, difficult to express or low in expression under normal conditions.
Service Advantages
- High success rate, up to 95%
- Prepare high-purity recombinant protein in a short time
- Mature design scheme and scientific screening system, select intracellular expression or secreted expression according to protein properties
- Provide a variety of expression cells (sf21, sf19 and Hi-5 cell lines)
- Achieve one-stop service from gene design to protein purification
- Competitive price in the market of protein expression and purification service
- Short service cycle, and urgent service is available
Workflow of Protein Expression and Purification at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of protein expression and purification, providing high quality of protein product, which can apply to protein folding, recrystallization and other experimental requirements. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality protein expression and purification solutions according to your needs and provide you with professional and thoughtful services.
References
- Murphy C I; Helen P W. UNIT 5.4 Overview of the baculovirus expression system. Berkeley Journal of International Law, 2010, 28(4): 91-93.
- Gómez A; et al. Protein production using the baculovirus-insect cell expression system. Biotechnology Progress, 2014, 30(1).
- Murphy C I; et al. Overview of the baculovirus expression system. Curr Protoc Mol Biol, 2004, chapter 4(1): 5.4.1-5.4.4.