Lentiviral Vectors Service
There is growing interest in cell and gene therapy, so there is a need for a more cost-effective and scalable lentiviral vectors production platform. Lentiviral vector gene therapy is widely used due to its combination of advanced clinical trials and regulatory approved therapies, as well as technical improvements in characterization and safety analysis. As a global leader in scientific services, Creative BioMart Vir-Sci' innovative solutions are designed to accelerate the development of genetically modified cell therapies and gene therapy. Through solutions covering the entire lentiviral workflow, we provide superior products, services and expertise that can help companies develop breakthrough lentiviral gene therapies.
Lentivirus
Lentiviral vectors (LVs) are viral vector systems based on the HIV-1 virus, which can efficiently introduce the gene of interest (or RNAi) into animal and human primary cells or cell lines. The genome of the lentiviral vector is positive-stranded RNA. After its genome enters the cell, it is reversed into DNA by its own reverse transcriptase in the cytoplasm to form a DNA integration complex. After entering the nucleus, the DNA is integrated into the cell genome. The integrated DNA transcribes the mRNA and returns to the cytoplasm to express the protein of interest or produce RNAi interference. Lentiviral vector-mediated gene expression or RNAi interference is persistent and stable because the target gene integrates into the host cell genome and divides as the cell genome divides. In addition, lentiviral vectors can effectively infect and integrate into non-dividing cells. The above characteristics make lentiviral vectors have distinctive characteristics compared with other viral vectors, such as unintegrated adenoviral vectors, adeno-associated virus vectors with low integration rates, and traditional retroviral vectors that integrate only dividing cells.
Figure 1. Third-generation lentiviral vector. (Milone; et al. 2018)
Application of Lentiviral Vector:
- Transfer the target gene/RNAi gene into cells that are difficult to transfect, such as neuronal cells, stem cells or other primary cells.
- Transfer the target gene/RNAi gene into animal tissues in order to obtain long-term expression.
- Construct a cell line that stably expresses the target protein/RNAi, and then introduce it into animals using in vivo method.
- Gene therapy.
- Transgenic animals.
- Gene knockout.
- Pharmaceutical research: construction of cell lines expressing receptor proteins and research on the effects of drugs.
- Quickly establish cell lines that produce protein of interest, a very promising eukaryotic cell expression method.
Our Lentiviral Vector (LV) Service
- Creative BioMart Vir-Sci provides genome-wide lentiviral and lentiviral products, including lentiviral plasmids and lentiviral particles.
- Creative BioMart Vir-Sci provides lentiviral CRISPR vectors.
- To further meet the customized needs of gene delivery, we now provide customized lentiviral cloning and lentiviral production services, including high titer and large-scale production. Based on research applications including in vitro cell culture and in vivo animal research, a variety of lentivirus titers will be provided.
Our Advantages
- Creative BioMart Vir-Sci will continue to provide process and analytical development, clinical and commercial supply of viral vector drugs and pharmaceutical products, and regulatory support to enable our customers to bring new drugs to market.
- Our team can flexibly support each customer's unique product needs, schedule and regulatory approach.
- Through recent investments and planned expansions in our world-class facilities, Creative BioMart Vir-Sci can meet your current and long-term viral vector production needs.
Workflow of Virus Services at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci provides a wide range of customized lentivirus services at a reasonable cost and fast turnaround time, involving various viruses and technical methods. Our experienced scientists are ready to solve difficult cloning projects. If you need related services, please feel free to contact us. Our expert team will design high-quality lentiviral vector (LV) services according to your needs and provide you with professional and thoughtful services.
Our Related Services
- Adenovirus Vector Service
- Adeno-Associated Virus Vector Service
References
- Cockrell, A. S.; Kafri, T. Gene delivery by lentivirus vectors. Molecular Biotechnology. 2017, 36 (3): 184–204.
- Milone, M. C.; O'Doherty, U. Clinical use of lentiviral vectors. Leukemia: official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, 2018, 32(7): 1.