Adenovirus (Ad) Vector Service

Creative BioMart Vir-Sci is one of the recognized experts in the field of adenovirus (Ad) vector production. Adenovirus vectors have been increasingly used in clinical trials of gene therapy, becoming the most widely used and promising viral vector after retroviral vectors. We have many years of experience in adenovirus vectors and advanced production systems and equipment. We can provide fast and satisfactory adenovirus vector services according to customer needs.

Adenovirus

Adenovirus is a large molecule (36 kb) double-stranded non-enveloped DNA virus. It enters cells through receptor-mediated endocytosis, and then the adenovirus genome is transferred into the nucleus, remains extrachromosomal, and does not integrate into the host cell genome. Adenoviruses are non-enveloped linear double-stranded DNA viruses that are widely distributed in nature, with at least 100 serotypes. Its genome is about 36kb in length, with an inverted terminal repeat region (ITR) at each end, and the inside of the ITR is a viral packaging signal. There are four early transcripts (E1, E2, E3, E4) distributed in the genome to perform regulatory functions, and one late transcript is responsible for encoding the structural protein. The early gene E2 product is a trans factor and an essential factor for replication of late gene expression, and the early gene E1A and E1B products are also necessary for early gene expression such as E2. Therefore, deletion of the E1 region can cause abortion of the virus during the replication phase. E3 is a replication non-essential region, and its deletion can greatly expand the insertion capacity. Adenovirus vectors are mostly based on Type 5 (Ad5) and Type 2 (Ad2), with the addition of Type 55 (Ad55).

Figure 1. Schematic of the genome of adenovirus type 5 (Ad5) and Ad5-based vectors A: Ad5. (Toth; et al,2013)

Adenoviral vectors have high transgenic efficiency, and in vitro experiments are usually close to 100% transduction efficiency; they can transduce different types of human tissue cells, regardless of whether the target cells are dividing cells; it is easy to make high-titer viral vectors. Therefore, adenoviral vectors have been increasingly used in gene therapy clinical trials, becoming the most widely used and most promising viral vector after retroviral vectors.

Adenoviral Vector System

The most mainstream adenoviral vector systems are the AdEasy system and the AdMax system.

AdEasy system: Prokaryotic recombination greatly improves the efficiency of adenovirus recombination.
AdMax system: The Cre/LoxP system has been transformed into a eukaryotic adenovirus packaging system to further increase the convenience of operation, and at the same time, the titer is further improved than that of AdEasy.

Our Advantages

Wide range of hosts.
Transient expression.
Strong immunogenicity.
High efficiency of host cells infecting dividing or non-dividing cells.
Relatively high titers enable highly effective drug delivery.

Our Adenovirus Vector Service but Not Limit to:

Recombinant adenovirus construction service.
Adenovirus vector characterization service.
Adenovirus vector purification service.
Adenovirus-based cDNA expression library generation service.
Development of upstream bioprocess of adenovirus vector.
Development of downstream process of adenovirus vector.

Applications

Adenovirus vectors can express mammalian foreign genes at high levels.
A large number of animal experiments and clinical experimental studies have shown that by carrying therapeutic genes, adenovirus vectors have a positive effect on cardiovascular diseases.
With the help of adenovirus vectors 's high affinity for the nervous system, precise positioning of specific cell groups in the tissue can be achieved.

Workflow of Services at Creative BioMart Vir-Sci

workflow

Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of adenovirus vector, involving various viruses and technical methods. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality adenovirus vector service according to your needs and provide you with professional and thoughtful services.

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