Phage Display Library Construction Service

Creative BioMart Vir-Sci provides a one-stop service based on phage display technology for customers both in academia and industry, including phage display library construction services. With years of rich experience, our experts can offer efficient and high-quality phage display library construction services to meet the needs for customers.

Phage Display

The principle of phage display technology is to insert the DNA sequence of foreign protein or peptide into the appropriate position of the structural gene of phage coat protein, so that the exogenous gene can be expressed with the expression of coat protein without affecting the normal function of coat protein. Finally, the foreign protein or peptide is displayed on the phage surface in the form of fusion with coat protein. The technology is primarily used to display interested peptides, antibodies, scaffolds or other proteins on the surface of phage and then to screen constructed libraries from a variety of displayed phages. This technology establishes a direct physical link between the protein and the gene, allowing rapid screening of required antibodies.

First step for phage display is library construction. Sequences of nucleic acid used to construct the library can be synthesized artificially or by cDNA. cDNA is usually used to obtain sequence for library construction. Phage display library construction process is to extract mRNA, obtain template cDNA by reverse transcription, and then design appropriate primer PCR to obtain the required gene sequence. This gene is then cloned into a phage vector and transformed into a receptive cell with phage helper to form a display library. The main phage display systems used to build libraries are the single-stranded filamentous phage display system, λ phage display system, T4 phage display system and T7 phage display system. According to different target, libraries can be classified with cDNA library, antibody library, protein library and random peptide library.

Figure 1. Schematic diagram of insert foreign gene into phage to construct phagemid library

Phage Display Library Construction Service

Creative BioMart Vir-Sci provides customers with high-quality and efficient antibody library construction (including native antibody library, immunized antibody library, etc.) and peptide library construction services, and also provides personalized library customization services according to customer needs. We have advanced library construction technology and rich experience in phagemid library construction.

Service Advantages

• Various phage display systems, including M13 single-chain filamentous phage display system, T4, T7 and λ phage display systems
• Covers the construction of multiple libraries, including native antibody library, immunized antibody library, scFv antibody library, etc.
• Provide high efficiency and quality library construction service
• Ensure satisfactory library construction service according to the customers’ specific needs
• Reasonable price in the market of phage display library construction service

Workflow of Phage Display Library Construction Service at Creative BioMart Vir-Sci

Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of phage display library construction for antibodies or peptides. If you are seeking for related services, please feel free to contact us, our expert team will design phage display library construction solutions according to your needs and provide you with professional and thoughtful services.

References

1. Hammers, C. M.; Stanley, J. R. Antibody phage display: technique and applications. Journal of Investigative Dermatology. 2014, 134(2): 1-5.
2. Vaughan, T. J.; et al. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nature Biotechnology. 1996, 14(3): 309-314.
3. Clackson, T.; et al. Making antibody fragments using phage display libraries. Nature. 1991, 352(6336): 624-628.