Induced Pluripotent Stem Cell Service
Induced pluripotent stem cells (iPS cells) are one kind of pluripotent stem cell that can be directly produced from somatic cells. Creative BioMart Vir-Sci has developed highly optimized protocols for efficient generation of iPS cells with multiple kinds of viral vectors. We provide not only high quality retrovirus and lentivirus packaging service but also efficient virus-based transduction and reprogramming to obtain iPS cells.
Induced Pluripotent Stem Cell
The iPS cell technology, developed first by Shinya Yamanaka’s lab in 2006, unveils the potential of reprogramming somatic cells into pluripotent stem cells by introducing specific transcription factors. To date, many kinds of terminally differentiated cells, including fibroblasts, urine cells, peripheral blood mononuclear cells (PBMCs), hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs), have been successfully reprogrammed via this method. By virtue of similar pluripotent characterization to embryonic stem cells, iPS cells have been a very popular resource in both basic and regenerative medicine for developing disease models, drug discovery as well as cell-based therapies.
Creative BioMart Vir-Sci Provides the Following Induced Pluripotent Stem Cell Services for Customers:
- Retroviruses and Lentiviruses Service
- Lentiviral Plus Cre-Lox Service
- Virus Reprogramming Service
Retrovirus and lentivirus based transduction of reprogramming factors represent a canonical and efficient strategy for generating iPS cells. High titer virus particles thus are key factors during the process. Creative BioMart Vir-Sci is a worldwide leader in producing high quality retrovirus and lentivirus particles for scientists in both academia and industry. By using highly standardized methods, we permit smart design of viral transfer vector as well as rapid production of high-titer retroviruses and lentiviruses.
Permanent integration of transgenes always arises concerns affecting further application of iPS cells. Moreover, it has been reported that integrated transgenes may compromise the developmental capacity of iPS cells. In addition to providing high titer lentiviral particles, Creative BioMart Vir-Sci is able to design and produce Cre excisable lentiviruses to facilitate removal of transgenes after the reprogramming event, thus improving the safety of our reprogrammed iPS cells.
Creative BioMart Vir-Sci has accumulated years of experience in generating iPS cell lines from a variety of custom cell types. By using highly standardized and efficient protocols, we can induce high quality iPS cells with a very high success rate via viral-based delivery system. Both integrating (Retrovirus and Lentivirus) and nonintegrating (Adenovirus and Sendai Virus) methods are available according to the research goal.
Figure 1. Schematic illustration describing general applications of iPS cells.
Features of Our Services
- Custom iPSC service
- Expert scientific team
- Highly efficient
- Rigorous quality control
- Reasonable price and fast turnaround time
- One-stop service
By using cutting edge technologies, Creative BioMart Vir-Sci provides iPS cell services that can be customized to fit your needs. Please feel free to contact us, and our scientific team will design high-quality solution to achieve your goals.
References
- Takahashi, K.; Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006, 126(4): 663-676.
- Sommer, C. A.; et al. Induced pluripotent stem cell generation using a single lentiviral stem cell cassette. Stem Cells. 2009, 27(3): 543-549.
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