Virus TCID50 Assay Service
In addition to the plaque assay, TCID50 is another method that can be used for virus titering. Creative BioMart Vir-Sci is a recognized expert in virus titering service. We have a mature virus titering service platform that can provide rapid and satisfactory virus TCID50 assay service according to customer needs. In addition, we also provide services for the virus plaque assay.
TCID50 Assay
Virus titering refers to calculate the amount of virus in a sample through an experimental method. This is necessary in some virus-related industrial production and scientific research. There are many methods for virus quantification, and the most common one is plaque measurement. Plaque detection is a good method to determine the virus titer, but it is not suitable for all viruses. Fortunately, there are several alternative methods to make up for these test gaps, such as, virus TCID50 assay. TCID50 assay is to serially dilute the virus suspension, and inoculation into animals, chicken embryos or cells cultured as a monolayer. And then, make a curve of the lethality or cytopathy of animals, chicken embryos caused by each dilution. Finally, find the end point dilution that causes 50% animal or chicken embryo death or cytopathy. Because clinical studies involve the determination of lethal doses and viruses that cannot form plaque, this method is often used in clinical researches. When using this method for testing in monolayer cell, the host cell monolayer is first placed on the 96 well plate, and then a series of serially diluted virus dilutions are added. After incubation for a certain period of time, observe and record the percentage of cell death in different groups after infection, so as to obtain the virus titer of the sample through further analysis and processing. For TCID50 assay and plaque assay, there are differences in data processing between the two. The mathematical principles and calculation formulas involved in the data processing of the TCID50 assay are more complicated. The commonly used calculation formulas include Spearman-Karber and Reed-Muench method. When it is necessary to make good estimates and/or treatment differences, but exist situations such as lacking the experimental material or extensive replicate titer determinations are precluded, the TCID50 method is an appropriate choice. According to the existing experimental data, the relative error of the plaque assay is 10-100%, while the error of TCID50 is about 35%. TCID50 is widely used in experimental and diagnostic virology to detect viruses including HIV-1, influenza and human herpesvirus.
Fig 1. The TCID50 assay for enumerating filoviruses. (Smither, et al. 2013)
Our Advantages
- Ensure high efficiency and quality for virus titering services
- Professional data processing and analysis
- Competitive price in the market of virus titering services
- Suitable for multiple virus titration detection
- Ensure 24/7 online service
- Short feedback cycle
Workflow of Microbiology Services at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of virus titering, involving various viruses and technical methods. We guarantee that each specific experimental step in the virus TCID50 assay is strictly controlled to ensure high quality virus service. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality virus titering solutions according to your needs and provide you with professional and thoughtful services.
References
- Smither, J. S.; et al. Comparison of the plaque assay and 50% tissue culture infectious dose assay as methods for measuring filovirus infectivity. Journal of Virological Methods. 2013, 193(2): 565-571.
- Flint, S.J.; et al. "Virological Methods". Principles of Virology. ASM Press. 2009, ISBN 978-1-55581-443-4.
- Pankaj, K. Methods for rapid virus identification and quantification. Materials & Methods. 2013, 3: 207.