Peptide Library Screening Service
As a well-recognized expert at phage display library screening, Creative BioMart Vir-Sci offers our worldwide clients the high quality services through our excellent strategies. Creative BioMart Vir-Sci is one of the authoritative and recognized experts in peptide library screening service and engaged in offering quality and personalized services of peptide library screening to meet our clients’ project goals.
Introduction
Phage display, reported by Smith in 1985, is a technique that uses phages to express exogenous genes in vitro to display a variety of peptides or proteins on the surface of phage shell. Displayed polypeptide or protein can maintain a relatively independent spatial structure and biological activity to facilitate the identification and binding of target molecules. This technique can show the genotype and phenotype of the molecule together, greatly facilitating the functional screening of peptides or proteins with high specificity and strong affinity.
Phage display polypeptide library is a kind of large volume combinatorial library for screening specific binding ligands of target molecules. The random peptides in the library are rich in diversity and have good biological activity. After incubation for a certain period of the peptide on the solid phase, the unbound phages were washed off, and then bounded phages were removed by competing receptors or acid pickling. The eluted phages infected host cells were propagated and amplified for the next round of elution. The required peptides can be screened out from the library by affinity screening with multiple rounds of "adsorption-elution - amplification" enrichment process.
Phage display peptide libraries provide a new method for screening bioactive peptides. This method has been used to screen peptides from mimic sugar, screened peptides can induce the immune response of animals to the corresponding sugars and regulate the activity of some glycosidase. In common, lectins, as well as monoclonal antibodies that recognize specific sugar antigens are used for screening target molecules. Besides, anti-cancer drugs developed by phage display peptide screening can provide new strategy to treat tumor diseases.
Technology Advantages
- Large scale production
- Great control over the screen process
- Fast process
- Easy to screen a large diversity of peptide
Figure 1. Schematic diagram of peptide library screening
Service Advantages
- Provide high efficiency and quality peptide screening service
- Ensure satisfactory peptide screening service according to the customers’ specific needs.
- Reasonable price
- Ensure 24/7 online service and short feedback cycle
Workflow of Peptide Library Screening Service at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci provides peptide library screening services and uses the standard process recognized by the international academic community to satisfy all kinds of experiments. According to different demands, various of targets can be screened by our service. With years of rich experience, our experts can offer high-quality peptide library screening services to meet the needs for customers. We accept peptide library provided by clients, but also provide related services to synthesize and express the interested peptide.
If you are seeking for related services, please feel free to contact us. We are pleased to leverage our rich experience and advanced platform to provide the best service and the most qualified products for you.
References
- Lu, H.; et al. Application of laser capture microdissection to phage display peptide library screening. Oral Surgery Oral Medicine Oral Pathology Oral Radiology & Endodontology, 2004, 98(6): 692-697.
- Kuramoto, K.; et al. Phage-displayed peptide library screening for preferred human substrate peptide sequences for transglutaminase 7. Archives of biochemistry and biophysics, 2013, 537(1): 138.
- Devlin, J. J.; et al. Random peptide libraries: a source of specific protein binding molecules. Science, 1990, 249(4967): 404-6.
- Pini, A.; et al. Design and Use of a Phage Display Library. Journal of Biological Chemistry, 1998, 273(34): 21769-21776.