PVX Vector System Service
Creative BioMart Vir-Sci provides customers with virus-related services in the plant field, including PVX-based expression vector design services. Our team of experts uses highly standardized and efficient methods to help customers construct high-quality vectors for exogenous proteins expression.
PVX-Based Expression System
Potato virus X (PVX), a type specie of the potexvirus family, contains a rod-shaped positive-strand RNA genome encapsidated with approximately 1300 coat protein subunits. The genome of PVX encodes a total of five open reading frames (ORFs). The viral RNA-dependent RNA polymerase (RdRp), which is required for replication, is encoded by the first ORF. The other ORFs encode a group of three movement proteins (also known as the triple gene block) as well as the coat protein, which are necessary for virion assembly, cell to cell movement and systemic movement. Similar to the Tobacco Mosaic Virus, PVX has also been developed as a full length expression vector as well as a deconstructed vector. Multiple antigens and antibodies have been produced by both kinds of vectors.
Full Length PVX Expression Vector
Several strategies have been developed to design transient expression vectors based on the full length PVX. For example, duplicated subgenomic promoters can be inserted between the triple gene block and the coat protein to drive the expression of foreign proteins. In addition, genes of interest (GOI) can be expressed as a fusion protein linked to the N terminus of the coat protein via the self-cleaving 2A peptide.
Features of The Full Length PVX Expression Vector
- Genetic stability. No elements of the PVX are deleted, thus increasing the genetic stability of the vectors during the infection.
- Full length PVX vectors retain the triple gene block and coat protein so that both cell to cell movement and systemic movement can be achieved.
Deconstructed PVX Expression Vector
For deconstructed PVX vectors, both the coat protein gene and triple gene block are replaced with the GOI, which is under the control of a native subgenomic promoter. The deconstructed vectors are much smaller than the original PVX but incapable of cell to cell and systemic movement. Multiple strategies have been utilized to optimize the expression of exogenous proteins.
Features of The Deconstructed PVX Expression Vector
- High level of foreign proteins can be produced by virtue of the efficiency of agroinfiltration.
- Suppressor of gene silencing can be co-expressed to further elevate the production efficiency.
- Different PVX strains can be used to obtain the highest yield.
- The 5’ untranslated region (UTR) sequence can be added between the promoter and the GOI to improve the yield.
Figure 1. Simplified diagram of full length (A) and deconstructed (B) PVX expression vectors. TGB: triple gene block; SGP: subgenomic promoter; GOI: gene of interest; CP: coat protein; 2A: the self-cleaving 2A peptide sequence from foot-and-mouth disease virus (FMDV)
Service Advantages
- Smart design of PVX-based vector
- Highly optimized protocol
- Top quality vector with flexible production scale
- Strict QC testing
- Competitive price in the market
Workflow of Construction of PVX Based Vectors
The expert team in Creative BioMart Vir-Sci will always find the suitable solution to your research goal. Please feel free to contact us for further questions.
References
- Hefferon, K. L. Plant virus expression vectors set the stage as production platforms for biopharmaceutical proteins. Virology. 2012, 433(1): 1-6.
- Lacomme, C.; Chapman, S. Use of Potato Virus X (PVX)–Based Vectors for Gene Expression and Virus‐Induced Gene Silencing (VIGS). Current Protocols in Microbiology. 2008, 8(1): 16I. 1.1-16I. 1.13.