Phage Display Service
Phage display is a technology that fusion peptide or protein genes into phage coat gene by molecular biology methods, then fusion genes are expressed with phage vector, so that target peptide or protein is displayed at surface of phage coat. Phage display is widely used in drug research, antibody development and disease treatment.
Creative BioMart Vir-Sci has accumulated a wealth of experience in the field of library construction and screening, involving various antibody or peptide, as well as advanced technical platforms. Creative BioMart Vir-Sci has developed a range of proprietary technologies and reagents that have significantly improved the phage display services to meet our clients’ project goals.
Creative BioMart Vir-Sci Provides the Following Phage Display Services for Customers:
- Phage Display Library Construction Service
- Phage Display Library Screening Service
- Antibody Library Screening Service
- Peptide Library Screening Service
Creative BioMart Vir-Sci is professional in applying advanced phage display library construction, contains but not limited to peptide and protein libraries.
Creative BioMart Vir-Sci can develop different strategies for library screening according to customers’ needs.
Antibody libraries were screened according to the specific binding characteristics of antigens and antibodies.
Screened peptide libraries can be used for drug development, ligand screening, protein function analysis, nucleic acid binding tests, or inhibitor screening.
Creative BioMart Vir-Sci provides phage display services and uses the standard process recognized by the international academic community to satisfy all kinds of experiments. Our experts can offer high-quality phage display services to meet the needs for customers.
Main Process of Phage Display is as Follows:
- Sequence used to construct library is synthesized by cDNA that obtain by reverse transcription.
- Target gene cloned into phage vector and transformed into receptive cell to assemble phage.
- Assembled phages are incubation with specific surface.
- Bound phages are collected by elution while non-bound phage are washed off.
Within 3-5 cycles, target peptide or protein are enough for downstream needs.
Figure 1. Schematic diagram of phage display
Workflow of Phage Display Service at Creative BioMart Vir-Sci
If you are seeking for related services, please feel free to contact us. We are pleased to leverage our rich experience and advanced platform to provide the best service and the most qualified products for you.
References
- Hammers, C. M.; Stanley, J. R. Antibody Phage Display: Technique and Applications. Journal of Investigative Dermatology, 2014, 134(2): 1-5.
- Clackson, T.; et al. Making antibody fragments using phage display libraries. Nature, 1991, 352(6336): 624-628.
- Hawkins, R. E.; et al. Making Antibodies by Phage Display Technology. Annual Review of Immunology, 1994, 12(1): 433.
- Pini, A.; et al. Design and Use of a Phage Display Library. Journal of Biological Chemistry, 1998, 273(34): 21769-21776.
- Hoogenboom, H. R. Selecting and screening recombinant antibody libraries. Nature Biotechnology, 2005, 23(9): 1105-1116.
Related Services: