Adeno-associated Viruses Packaging Service
Creative BioMart Vir-Sci provides adeno-associated viruses packaging services and uses the standard process recognized by the international academic community to satisfy all kinds of experiments. We have years of viral vector development and viral packaging experience to provide fast and satisfactory adeno-associated viruses packaging services according to customer needs.
Adeno-associated Viruses Packaging
Adeno-associated virus (AAV) is a kind of nonpathogenic viruses with single-stranded DNA, it relies on helper viruses such as adenovirus or herpes simplex viruses for replication. The genome of AAV is approximately 4800bp in length, consisting of two inverted terminal repeats (ITRs) with size of 145bp and two open reading frames (ORFs, Rep and Cap). ITRs play a decisive role in virus packaging, and ORFs can regulate the expression of various proteins, such as Rep78, Rep68, Rep52, Rep40. AAV has been widely used in basic research and clinical trials due to its wide host range, high safety, low immunogenicity, stable expression and stable physical properties. AAV vectors have become one of the most commonly used gene therapy vectors in the world.
Figure 1. Genetic Schematic Diagram of Adeno-associated viruses
At present, the commonly used AAV packaging system is AAV helper free system, which contains one transfer plasmid, one Rep-Cap expression plasmid and one helper plasmid. The ITR sequence in the transfer plasmid carrying the target gene comes from AAV2. Recombinant AAV packaging vector use target genes replacing Rep gene and Cap gene, and then co-transfected with plasmids expressing Rep and Cap genes to produce packaged AAV containing target genes. After cloning the target gene to the recombinant AAV vector, the adenovirus genes necessary for AAV infection (E2A, E4 and VA) were cloned to plasmid, and then the Rep gene and Cap gene were constructed into plasmid.
Adeno-associated Viruses Packaging Services
Creative BioMart Vir-Sci has developed a range of proprietary technologies and reagents that have significantly improved the AAV packaging services process in terms of virus titer, purity, activity and consistency, especially for the helper free system used in our vector construction services. Combining our online vector design platform, offline vector and virus production line, we can continuously provide high-quality customized products of AAV.
Technology Advantage
- High security. So far, no wild-type AAV has been found to be pathogenic to humans. In fact, recombinant AAV packaged by Creative BioMart Vir-Sci has removed most of genomic elements of wild-type AAV and further ensured its security.
- Broad host range. AAV can infect both dividing cells and non-dividing cells.
- Stable expression. The AAV packaged by Creative BioMart Vir-Sci can mediate the long-term stable expression of foreign genes.
- Stable properties. Recombinant AAV can be stored at 4oC for one week and can not be inactivated at 60oC, it is also responsible for the relative resistance of chloroform.
- Low immunogenicity. The AAV genome only contains 4681 nucleotides, which is easy to package and the immune response is low in animal experiments.
Service Advantages
- Ensure high efficiency, high quality and strict preparation process for AAV packaging services.
- Competitive price in the market of AAV packaging services.
- Ensure 24/7 online service.
Workflow of Adeno-associated Viruses Packaging at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of AAV packaging, providing high quality of AAV based on the AAV helper free system, which can apply to preclinical cytological and holistic animal experiments. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality AAV packaging solutions according to your needs and provide you with professional and thoughtful services.
References
- Dalkara, D.; et al. In vivo-directed evolution of a new adeno-associated virus for therapeutic outer retinal gene delivery from the vitreous." Sci Transl Med. 2013, 5: 189.
- Xie, Q.; et al. The atomic structure of adeno-associated virus (AAV-2), a vector for human gene therapy. Proc Natl Acad Sci U S A. 2002, 99(16): 10405-10410.
- Wu, Z.; et al. Adeno-associated virus serotypes: vector toolkit for human gene therapy. Mol Ther. 2006, 14(3): 316-327.