TMV RNA-Based Overexpression (TRBO) Vector Service
As a worldwide leader in the field of viral services, Creative BioMart Vir-Sci provides high-quality viral vector services for scientists in the plant field, including TMV RNA-based overexpression (TRBO) vectors. By using highly standardized and optimized protocols, we produce high quality vectors used for flexible expression of recombinant proteins.
TMV RNA-based Overexpression Strategy
Tobacco mosaic virus RNA-based overexpression (TRBO) strategy represents a breakthrough in the development of plant expression systems. Briefly, the single module TRBO vector contains the 35S promoter driven replicon that derived from the TMV genome. In addition, the coat protein gene is replaced with a multiple cloning site where the genes of interest (GOI) could be inserted. Typically, the GOI is under the control of a native subgenomic promoter. As a transient expression system, the TRBO vector has been used worldwide due to enormous advantages such as ease of use, high protein expression levels and increased biosafety.
Figure 1. Simplified diagram of the TRBO vector. 35S: Cauliflower Mosaic Virus 35S promoter, MP: movement protein, SGP: subgenomic promoter, CaMV: Cauliflower Mosaic Virus PolyA terminator.
Technology Advantage
- The TRBO vector can be delivered via agroinfection with a very high rate, which enables infection of multiple plant tissues even without systemic movement of the vector.
- The deletion of coat protein makes it easier to purify foreign proteins from infected tissues.
- Diluted culture of A. tumefaciens can be used to achieve robust infections.
- Significantly high protein expression level: up to 5.5g of recombinant GFP/kg of infiltrated tissue can be obtained.
- The TRBO vector cannot generate virions in plants, which greatly increase biosafety.
Service Advantages
- Smart design of TRBO vector
- Highly optimized protocol and experienced team
- Top quality vector with flexible production scale
- Strict sequencing test
- Competitive price in the market
- Short turnaround time and urgent services are available
Workflow of TMV RNA-Based Overexpression (TRBO) Vector Construction Service
The TRBO vector service provided by Creative BioMart Vir-Sci enables you to focus on your research interests. Simply provide your vector design needs to our experienced experts, and we will use highly-optimized protocols to construct a TRBO vector for you within a short period of time. Please feel free to contact us if you have any questions.
References
- Lindbo, J. A. TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. Plant physiology. 2007, 145(4): 1232-1240.
- Peyret, H.; Lomonossoff, G. P. When plant virology met Agrobacterium: the rise of the deconstructed clones. Plant biotechnology journal. 2015, 13(8): 1121-1135.