Recombinant Adenoviruses Amplification Service

Creative BioMart Vir-Sci is one of the authoritative and recognized experts in recombinant adenoviruses amplification service. We have rich experience in construction and amplification of recombinant adenovirus amplification service. Besides, our complete equipment enables us to provide high-quality recombinant adenovirus amplification services according to customers’ needs.

Introduction

Adenoviruses are non-enveloped linear double-stranded DNA viruses that are widely distributed in nature, with at least 100 serotypes. Recombinant adenoviruses are products obtained by genetic modification of adenovirus. The polymerase chain reaction technology is used to amplify the target gene onto the shuttle vector, and then the recombinant shuttle vector is homologous recombined with the adenoviruses to obtain recombinant adenoviruses. In recent years, recombinant adenoviruses as a carrier vaccine have been widely used in scientific research and clinical trials. Many research showed that recombinant adenoviruses have a significant effect in killing tumors and cancers.

Figure 1. Novel system for recombination adenovirus vector production. (Sato-Dahlman, et al. 2018)

Reasons to construct recombinant adenoviruses:

• Increase the loading capacity of foreign genes. Many gene therapy programs are limited by the low loading capacity of adenoviruses. In order to further expand the application of adenovirus vectors, it is necessary to delete some trans-acting elements in their genomes to increase their loading capacity.
• Reduce immunogenicity. The strong immunogenicity of adenoviruses is mainly caused by its capsid protein and late gene expression products (L1 - L5). Therefore, it is possible to reduce the immunogenicity of adenoviruses by controlling the expression of its late genes.
• Improve targeting. The wide range of adenoviruses hosts makes it widely used in various fields of gene therapy. However, in many gene therapy programs, it is only necessary to infect specific tissues or cells.

Amplification in 293 cells

Through genetic engineering technology and gene recombination technology, the required recombinant adenovirus is constructed, and the positive recombinant adenoviruses are screened after identification. The 293 cells belong to the human renal epithelial cell line, and there are a variety of derivative strains, which are relatively easily transfected. Therefore, the 293 cells are commonly used to express foreign genes for scientific research or gene therapy. The 293 cells are also the most suitable host cells for recombinant adenoviruses amplification.

Our recombinant adenoviruses amplification service:

• Constructing recombinant adenoviruses
• Screening for positive recombinant adenoviruses
• Amplifying recombinant adenoviruses

Our Advantages

• Provide high efficiency and quality recombinant adenoviruses amplification service
• Ensure satisfactory amplification service according to the customers’ specific needs
• Suitable for amplification of multiple recombinant adenoviruses
• Reasonable price in the market of recombinant adenoviruses amplification service
• Ensure 24/7 online service and short feedback cycle

Workflow of Microbiology Services at Creative BioMart Vir-Sci

Creative BioMart Vir-Sci has accumulated a wealth of experience in the field of recombinant adenoviruses amplification service, involving various viruses and technical platforms. We guarantee that each specific experimental step is strictly controlled to ensure high quality of recombinant adenoviruses amplification. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality recombinant adenoviruses amplification solutions according to your needs and provide you with professional and thoughtful services.

References

1. Sato-Dahlman, M.; et al. The Development of Oncoltyic Adenovirus Therapy in the Past and Future - For the Case of Pancreatic Cancer. Current cancer drug targets. 2018,18(2): 153-161.
2. Liu, L.; et al. Therapeutic efficacy of an hTERT promoter-driven oncolytic adenovirus that expresses apoptin in gastric carcinoma. International journal of molecular medicine. 2012, 30(4): 747-754.
3. Qi, Y.; et al. Preclinical pharmacology and toxicology study of Ad-hTERT-E1a-Apoptin, a novel dual cancer-specific oncolytic adenovirus. Toxicology and Applied Pharmacology. 2014, 280(2): 362–369.