Lentiviruses Packaging Services

Creative BioMart Vir-Sci provides lentiviruses packaging services and uses the standard process recognized by the international academic community to satisfy all kinds of experiments. We have years of viral vector development and viral packaging experience to provide fast and satisfactory lentiviruses packaging services according to customer needs.

Lentivirus

Lentiviruses are a type of retroviruses with a double-stranded RNA genome. The genome of lentivirus has a complex structure, with three similar genes (gag, pol and env) to retrovirus, four helper genes (vif, vpr, nef and vpu) and two regulatory genes (tat and rev). However, different from common retroviruses, lentiviruses have a wider range of hosts and are capable of infecting both mitotic and non-mitotic cells. Recombinant lentivirus vector is a tool vector based on HIV-1 (human immunodeficiency virus type I), and its virulence genes have been removed and replaced by exogenous target genes. Lentivirus systems have been widely used in gene overexpression, RNA interference, microRNA research of various cell lines and live animal experiments.

Figure 1. Genetic Schematic Diagram of HIV-1 lentivirus

Lentivirus expression vector contains the genetic information needed for packaging, transfection, and stable integration. Lentivirus packaging plasmids provide all accessory protein needed for the recombinant pseudo-viral vector. The target gene enters the host cell, it is reversely transcribed and integrated into the genome, thus expressing high levels of effector molecules. Combining our online vector design platform, offline vector and virus production line, we can continuously provide high-quality customized products of lentivirus.

Our Lentiviruses Packaging Services

Creative BioMart Vir-Sci uses two mainstream and optimized methods to package lentiviruses according customer needs.

• Tri-plasmid Packaging

The third-generation lentiviral packaging system used by Creative BioMart Vir-Sci includes one transfer plasmid, one envelope plasmid and two packaging plasmids (Gag/Pol expression plasmid and Rev expression plasmid). Envelope plasmid expressed vesicular stomatitis virus G (VSV-G) protein, lentivirus vector with VSV-G protein expanded target cell tropism range, increased the stability of the vector, and concentrated the vector by high-speed centrifugation, thus improving the titer of viruses. In addition to the cis sequence required for packaging, reverse transcription and integration, the transfer plasmid retains gag and RRE with length of 350 bp and inserts the target gene into it. Creative BioMart Vir-Sci uses the third-generation lentiviral packaging system to provide lentiviruses in a short service period, and at the same time improve its biosecurity as much as possible.

• Four Plasmid Packaging

In order to reduce the sequence homology of HIV-1 packaging structure and reduce the possibility of recombination into RCV, four plasmid expression system removed the auxiliary genes. Because the transduction of gag-2-pol needed rev, Creative BioMart Vir-Sci adds a plasmid containing REV to the tri-plasmid system, thus reducing the possibility of RCV production without affecting the non-mitotic cell transduction efficiency.

Technology Advantage

• Long expression time. By integrating exogenous genes into the host cell genome, lentiviruses can achieve stable expression of target genes for a long time without losing them with cell division and passage, which are the first choice for cell experiments.
• Infection with a wide range. Packaged lentiviruses can effectively infect dividing and non-dividing cells, and are suitable for almost all cell lines, such as neuronal cells, hepatocytes, cardiomyocytes, tumor cells, endothelial cells and stem cells.
• High security. Pathogenicity has not been found and lentiviruses have been widely used in CAR-T therapy in humans.
• Low immunogenicity. Direct injection of packaged lentiviruses into living tissue generally does not cause an immune response, so it is suitable for animal experiments.

Service Advantages

• Provide lentivirus packaging services with different titers (low titer, high titer, ultra-high titer) and different scales (small scale, medium scale, large scale)
• Ensure high efficiency, high quality and strict preparation process for lentiviruses packaging services
• Competitive price in the market of virus packaging services
• Ensure 24/7 online service

Workflow of Lentiviruses Packaging at Creative BioMart Vir-Sci

Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of lentiviruses packaging, providing high quality of lentiviruses based on different plasmid packaging systems, which can apply to preclinical cytological and holistic animal experiments. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality lentiviruses packaging solutions according to your needs and provide you with professional and thoughtful services.

References

1. Tiscornia, G.; et al. Production and purification of lentiviral vectors. Nat Protoc. 2006, 1(1): 241-245.
2. Dull, T.; et al. A third-generation lentivirus vector with a conditional packaging system. J Virol. 1998, 72(11): 8463-8471.
3. Van Maele, B.; et al. Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction. J Virol. 2003, 77(8): 4685-4694.
4. Andrew, M.; et al. Lentiviral vectors. J Biomed Sci. 2004, 11: 439-449.