Recombinant VSV Amplification Service
Creative BioMart Vir-Sci is one of the authoritative and recognized experts in recombinant vesicular stomatitis viruses (rVSV) amplification service. We have extensive experience in providing recombinant vesicular stomatitis viruses amplification service. Besides, our complete equipment enables us to provide high-quality recombinant vesicular stomatitis viruses amplification services according to customers’ needs. Moreover, we also provide amplification services for recombinant adenovirus.
Recombinant Vesicular Stomatitis Viruses
Vesicular stomatitis virus (VSV) is a negative-strand RNA virus with no segments, and its genome is about 11 kb in length. The packaging of negative-strand RNA viruses requires that RNA and nucleocapsid protein form an active ribonucleoprotein, which serves as a template for RNA-dependent RNA polymerase, so pure negative-strand RNA is not infectious and the DNA will not appear in the virus replication cycle. Furthermore, the viral genome will not be integrated into the host cell genome, so the rVSV vector vaccine derived from the modified VSV has higher safety. rVSVs are products obtained by genetic modification of VSVs, which can be used to prepare vaccines and treat tumors. rVSVs as carrier vaccines have been studied for many years. They have been used in human immunodeficiency virus, influenza virus, Nipah virus, Ebola virus, etc. Among which the Ebola vaccine with rVSV as the carrier has completed the phase III clinical trial and submitted to the US FDA for approval.
Figure 1. Schematic representation of the production of HCVrv. (Tani, et al. 2007)
Recombinant Vesicular Stomatitis Viruses Amplification
In order to reduce the toxicity of wild-type VSV, or overexpress foreign proteins, such as targeted mutant protein M, the rVSVs are produced through genetic engineering technology. The amplification of rVSV is slightly more complicated than other viruses. The amplification process involves the materials such as recombinant plasmid carrying rVSV genome, helper plasmid pBS, poxvirus, cultured cells, etc. Since different cell lines have different effects on the packaging efficiency and expansion of rVSV, it is very important to choose the appropriate packaging system. During the amplification of rVSV, different packaging systems are usually screened, such as 293T cells, BHK-12 cells, Vero cells, etc.
Our Recombinant Vesicular Stomatitis Virus Amplification Service
- Constructing rVSV
- Screening for positive rVSV
- Amplifying rVSV
Our Advantages
- Provide high efficiency and quality rVSVs amplification service
- Ensure satisfactory amplification service according to the customers’ specific needs
- Suitable for amplification of multiple rVSVs
- Reasonable price in the market of rVSVs amplification service
- Ensure 24/7 online service and short feedback cycle
Workflow of Microbiology Services at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci has accumulated a wealth of experience in the field of recombinant virus amplification service, involving various viruses and technical platforms. We guarantee that each specific experimental step is strictly controlled to ensure high quality of rVSVs amplification. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality rVSVs amplification solutions according to your needs and provide you with professional and thoughtful services.
References
- Pol, A. N. V. D.; et al. Chikungunya, influenza, nipah, and semliki forest chimeric viruses with vesicular stomatitis virus: actions in the brain. Journal of Virology. 2017,91(6): e02154-16.
- Dolzhikova I. V.; et al. Safety and immunogenicity of GamEvac-Combi, a heterologous VSV- and Ad5-vectored Ebola vaccine: an open phase I/II trial in healthy adults in Russia. Human vaccines and immunotherapy. 2017, 13(3): 613-620.
- Gsell P. S.; et al. Ring vaccination with rVSV-ZEBOV under expanded access in response to an outbreak of Ebola virus disease in Guinea, 2016: an operational and vaccine safety report. Lancet Infectious Disease. 2017, 17(12): 1276-1284.
- Tani, H.; et al. Replication-competent recombinant vesicular stomatitis virus encoding hepatitis C virus envelope proteins. Journal of Virology. 2007, 81(16): 8601-8612.