Virus-induced Gene Silencing Service
Creative BioMart Vir-Sci is well-known in production of virus-induced gene silencing. We have extensive experience in providing virus-induced gene silencing service. Besides, our complete equipment enables us to provide high-quality virus-induced gene silencing services according to customers’ needs.
Virus-induced Gene Silencing
Virus-induced gene silencing (VIGS) was first used to describe the recovery phenomenon of virus-infected plants, it is an RNA-mediated antiviral defense mechanism of plant, and has been developed as a reverse genetics tool for gene function analysis recently. VIGS refers to the virus carrying the target gene fragment infecting the plant, which can induce the silencing of homologous genes in the plant, thereby helping researchers to analyze the function of the target gene through the study of plant phenotype changes. VIGS includes three important steps, inserting the target gene fragments to be silenced into the viral genome, infecting a suitable host plant, and finally using the plant's defense mechanism against virus infection to silence the target gene. Compared with traditional methods of studying plant gene blocking such as gene knockout, and antisense suppression, VIGS has the characteristics of simplicity, efficiency, short periodicity, no need to transform plants, and the ability to silence gene families, etc., and its silencing effect can be passed on to future generations under certain conditions. Therefore, VIGS has been widely used in the functional identification of genes related to physiological pathways such as plant disease resistance and stress resistance, growth and development, and metabolic regulation.
Figure 1. The model of virus-induced gene silencing (Waterhouse, et al. 2003)
Application of VIGS
Plant Gene Function
Plant Anti-Virus
The most common use of VIGS technology is to identify plant functional genes. VIGS can induce target gene silencing in plant tissues and organs. VIGS technology is a fast and effective method for studying plant gene function, especially for some non-model plant gene functions that are difficult to transform.
Through virus-induced gene silencing, transgenic plants can exhibit antiviral properties. The new vector and new inoculation method make VIGS technology applicable to some dicotyledonous species.
Our Virus-Induced Gene Silencing Service
- Plant viral vectors construction Service
- Plant transfection service
- Transgenic plant culture service
Our Advantages
- Provide high efficiency and quality plant viral vectors construction service according to the customers’ specific needs
- Ensure professional plant transfection and transgenic plant culture technology
- Reasonable price in the market of virus-induced gene silencing service
- Ensure 24/7 online service and short feedback cycle
Workflow of Virus-induced Gene Silencing Service at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci has years of experience in the field of virus-induced gene silencing service. We guarantee that each specific experimental step is strictly controlled to ensure high quality of virus-induced gene silencing technology. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality virus-induced gene silencing solutions according to your needs and provide you with professional and thoughtful services.
References
- Fu, D. Q.; et al. Enhancement of virus-induced gene silencing in tomato by low temperature and low humidity. Molecules and cells. 2006, 21(1): 153-160.
- Fu, D. Q.; et al. Virus-induced gene silencing in tomato fruit. Plant Journal. 2005, 43(2): 299-309.
- Waterhouse, P. M.; et al. Exploring plant genomes by RNA-induced gene silencing. Natural Reviews Genetics. 2003, 4(1): 29-38.