Plant Viral Vectors Construction Service

Creative BioMart Vir-Sci owns years of experience in plant viral vectors construction. With advanced facilities and professional personnel, we can complete plant viral vectors construction in short time. In order to ensure quality, our vector products have undergone multiple verifications. We have a complete transportation system to deliver products to customers. Creative BioMart Vir-Sci strives to provide you with thoughtful service.

Plant viral vector construction refers to using plant virus to construct a vector that introduces foreign genes into plant cells and enables the genes to be efficiently expressed in host cells.

Plant Viral Vectors Construction Services

• Cloning of CP Gene

We first designed and cloned primers of appropriate length based on the full-length sequence of the CP gene of the virus, and evaluated the designed primers. Then we extract the RNA of the plant virus, and then obtain cDNA by reverse transcription. We use cDNA as a template to amplify the designed primers by PCR. Then recover and sequence the amplified product and compare it with the full sequence of the CP gene.

• Vectors Construction

We use overlap-PCR technology to splice the CP gene fragment and the target gene to construct a plant expression vector. Then we identified the constructed plant viral vectors.

Application

Plant viral vectors have a wide range of recipient plants, and require a short time for transformation, which are particularly suitable for the expression of a large number of foreign genes. In addition, exogenous genes mediated by plant virus vectors are not integrated into the host nuclear genome, but are proliferated and expressed in host cells following the invasion of the virus, which can be used to study genes and gene mutants of unknown function, and can also be used as a place where foreign proteins are processed to produce rare and hard-to-obtain proteins by conventional methods.

Service Advantages

• Short service cycle
• High-quality plant viral vector products
• Various types of plant viral vector construction services
• Reasonable and affordable price
• Thoughtful after-sales service

Workflow of Plant Viral Vectors Construction Service at Creative BioMart Vir-Sci

Creative BioMart Vir-Sci is a company that provides professional plant viral vector construction services. In the past few years, we have provided services to dozens of companies and institutions, and have won unanimous praise from them with our professional service and enthusiastic attitude. If you are looking for relevant service, please feel free to contact us. We will respond to you in time and offer you thoughtful service.

Reference

1. John, A. L. TRBO: A high-efficiency tobacco mosaic virus RNA-based overexpression vector. Plant Physiology, 2007,145(4): 1232-1240.

Related Services:

• Tobacco Mosaic Virus (TMV) Vector System Service
• TMV RNA-Based Overexpression (TRBO) Vector Service
• PVX Vector System Service
• Plantago Asiatica Mosaic Virus (PlAMV) Vector System Service
• Foxtail Mosaic Virus (FoMV) Vector System Service
• CPMV-Based Expression Vector System Service