Virus Packaging Services
Creative BioMart Vir-Sci provides retroviruses packaging service, lentiviruses packaging service, adenoviruses packaging service and adenoviruses packaging service. We have years of viral vector development and viral packaging experience to provide fast and satisfactory virus packaging services according to customer needs. The quality control of our virus packaging services adopts the standard process recognized by the international academic community, which can completely satisfy all kinds of experiments.
Virus Packaging
Virus packaging is a kind of genetic diagnosis and gene therapy technique, which packages foreign genes into the shell of the natural virus. Using the virus infectivity to target cells, our virus packaging services introduce foreign genes into the target cell and change the expression of foreign genes in target cells. Some abnormal genes (such as oncogenes and viral genes) can be suppressed by introducing foreign fragments through antisense nucleic acid technology. Some genes have therapeutic effects of their own, which can be synthesized in vitro and introduced into the body to increase their expression.
Our Virus Packaging Services
- Retroviruses Packaging Service
- Lentiviruses Packaging Service
- Adenoviruses Packaging Service
Retroviruses are a class of single-stranded RNA viruses that contain reverse transcriptase. Retroviruses are suitable for mediating the long-term stable expression of foreign genes in host cells. After the invasion to the host cell, the retrovirus takes its own RNA as the template and forms a DNA loop by the reverse transcriptase, and then integrates the DNA loop into the chromosomes of host cells and completes replication in host cells as a provirus. We offer packaging services for MMLV retroviruses and MSCV retroviruses.
Lentiviruses are RNA viruses and belong to the retrovirus family, lentiviral vectors can be used for gene therapy based on HIV-1 (human immunodeficiency virus type I). Different from retroviral vectors, lentiviral vectors have the ability to infect both dividing cells and non-dividing cells, which can effectively integrate exogenous genes into chromosomes of host cells to achieve persistent expression. Lentiviruses are widely used in the study of RNAi expression. In general, siRNA synthesized in vitro is transient in inhibiting gene expression, which limits its application. However, the intracellular transcription of siRNA based on lentiviral vector not only increases the number of effectively transfected cells, but also stably inhibits the expression of the target gene in cells for a long time. It can effectively infect neurons, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, stem cells and other cells, and has a good gene therapy effect and broad application prospects.
Adenovirus are non-enveloped particles with diameters of 70-90nm, consisting of 252 capsomeres with diameters of 7-9nm arranged in icosahedral form. The capsid of adenovirus contains linear double-stranded DNA molecules with a length of 35,000bp, and there are reverse repeats of about 100bp at each end. The structural proteins of adenovirus accumulate in the nucleus to form viral capsids, and the viral genome is packed to form infectious virus particles, which eventually lyse host cells and are released to complete the life cycle of adenovirus. By removing E1 and E3 associated with virus coding protein, recombinant adenovirus system can bind to host cells of trans-complementary virus E1 protein, which not only retains the advantages of high transduction efficiency and high expression level of adenovirus itself, but also eliminates the risk of self-replication of progeny virus and reduces immunogenicity, making it an excellent gene transmission tool.
Figure 1. Structure of Adenovirus. (Russell, 2009)- Adeno-associated Viruses Packaging Service
Adeno-associated viruses are non-enveloped particles with single-stranded DNA and belong to parvovirus family. The genome of adeno-associated virus, approximately 4700bp, consists of two upstream and downstream open reading frames (ORFs), located between two inverted terminal repeats (ITRs) of 145 nucleotides. Because of its wide range of host, high safety, low immunogenicity, stable expression and physical properties, adeno-associated viruses have been widely used in basic research and clinical trials.
Service Advantages
- Ensure high efficiency, high quality and strict preparation process for virus packaging services
- Competitive price in the market of virus packaging services
- Ensure 24/7 online service
Workflow of Virus Packaging Service at Creative BioMart Vir-Sci
Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of virus packaging, providing high quality of retroviruses, lentiviruses, adenoviruses and adeno-associated viruses, which can apply to preclinical cytological and holistic animal experiments. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality virus packaging solutions according to your needs and provide you with professional and thoughtful services.
References
- He, T.C.; et al. A simplified system for generating recombinant adenoviruses. Proceedings of the National Academy of Sciences. 1998, 95(5): 2509-2514.
- Morita, S.; et al. Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Therapy. 2000, 7: 1063-1066.
- Russell, W.C. Adenoviruses: update on structure and function. Journal of General Virology. 2009, 90(1): 1-20.
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