Adenoviruses Packaging Service

Creative BioMart Vir-Sci provides adenoviruses packaging services and uses the standard process recognized by the international academic community to satisfy all kinds of experiments. We have years of viral vector development and viral packaging experience to provide fast and satisfactory adenoviruses packaging services according to customer needs.

Adenoviruses Packaging

Adenovirus vectors have been widely used in gene therapy and vaccine research because of its high loading capacity, high expression speed and high viral titer. During the packaging process of adenovirus vectors, the exogenous DNA fragment located between two inverted repeat sequences is further packaged into virus particles with viral proteins expressed by the packaged cell. The genome of adenovirus encodes multiple structural proteins and functional proteins, for example, E1, E2, E3 and E4 protein. Generally, first-generation adenovirus vectors remove E1 or E3 genes and can trigger strong inflammatory and immune responses in the body without purification. After purification, it can be safely used with an expression cycle of up to 4 weeks in vivo. Second-generation adenovirus vectors remove E2 or E4 genes and have a weaker immune response. Third-generation adenovirus vectors remove all or most of related genes (gutless adenovirus vector or mini adenovirus vector), and only ITRs and packaging signal sequences are retained. This vector system can insert genes with a maximum length of 35KB, and the cellular immune response caused by viral protein is further reduced. At the same time, the transduction of genes located in nuclear matrix attachment region into the vector can maintain the long-term expression of foreign genes, and increase the stability of the vector.

Fig. 1 Genetic Schematic Diagram of Adenovirus

Adenoviruses Packaging Services

Creative BioMart Vir-Sci has developed a range of proprietary technologies and reagents that have significantly improved the adenovirus packaging process in terms of virus titer, purity, activity and consistency, especially for the first adenovirus vector system used in our vector construction services. Combining our online vector design platform, offline vector and virus production line, we can continuously provide high-quality customized products of adenovirus.

Technology Advantage

• Lower risk. After transduction into target cells, adenovirus vector maintains the state of free DNA in the nucleus, which can reduce the risk of cancer caused by the destruction of host genome.
• Large capacity. In theory, the maximum genome size that adenovirus can effectively package is about 38.7 KB (from 5' ITR to 3' ITR). Removing the major components required for adenovirus packaging expression, our vector has a large capacity of about 7.5 KB to accommodate the target DNA (such as promoters, ORFs, and fluorescent markers), which can meet most application needs.
• High titer. Adenoviruses can be massively amplified by re-transducing the packaged cells into obtain viruses of ultra-high titer, which are not present in either lentivirus vectors or retrovirus vectors.
• Broad host range. Adenovirus vectors can transduce cells derived from commonly used mammals such as humans, mice and rats. In addition, our vectors can not only be used in live animal experiments, but also has the ability of cell transduction in vitro.
• High security. To ensure the biological safety of produced adenovirus vectors, we removed genes necessary for virus packaging, and these genes are integrated into the genome of the packaged cell. These replication-deficient viruses can replicate only in packaged cells.

Service Advantages

• Ensure high efficiency, high quality and strict preparation process for adenoviruses packaging services.
• Competitive price in the market of virus production services.
• Ensure 24/7 online service.

Workflow of Adenoviruses Packaging at Creative BioMart Vir-Sci

Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of adenoviruses packaging, providing high quality of adenoviruses based on the first-generation plasmid packaging systems, which can apply to preclinical cytological and holistic animal experiments. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality adenoviruses packaging solutions according to your needs and provide you with professional and thoughtful services.

References

1. Bett, A.J.; et al. An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proc Natl Acad Sci U S A. 1994, 91(19): 8802-8806.
2. Duigou, G.J.; Young, C.S. Replication-competent adenovirus formation in 293 cells: the recombination-based rate is influenced by structure and location of the transgene cassette and not increased by overproduction of HsRad51, Rad51-interacting, or E2F family proteins. J Virol. 2005, 79(9): 5437-5444.