Baculovirus Vector Construction Service

Creative BioMart Vir-Sci provides vector construction services and uses the standard process recognized by the international academic community to satisfy all kinds of experiments. We have years of vector construction experience in baculovirus/insect expression system to provide fast and satisfactory services according to customer needs.

Baculovirus Expression System

The baculovirus expression system is a popular method for the production of recombinant proteins. Baculovirus shuttle vector (bacmid) contains a mini-F replicon, which allows autonomous replication and stable segregation of plasmids at low copy number, a selectable kanamycin resistance marker, and attTn7, the target site for the bacterial transposon Tn7, all inserted into the polyhedrin locus of Autographa califomica nuclear polyhedrosis virus (AcNPV). Bacmid can replicate in E. coli as a large plasmid, and it is still infectious after being introduced into insect cells. Recombinant bacmid has been widely used for protein expression of viruses, fungi, plants, and animals.

Figure 1. Schematic diagram of recombinant bacmid selection and transfection of insect cells.

The donor baculovirus vector with target gene is transformed into E. coli DH10Bac. Bacmid with transposition was inactivated due to the insertion of LacZ gene, making recombinant bacmid colony colorless, then recombinant bacmid DNA was extracted and used for transfection of insect cells Sf9.

Technology Advantage

• Easy to operate. Baculovirus genome is small, with relatively simple molecular biological characteristics and a variety of restriction enzyme sites.
• Baculovirus virions are rod-shaped and have the plasticity to accommodate large foreign genes.
• p10 and polyhedron in baculovirus are highly transcribed during the late stages, their promoter can express the target protein efficiently.
• The host of baculovirus is insect cells, and the target protein can be processed and modified by the post-translational modification system of insect cells.
• High security. Baculovirus expression vector is very specific and can only replicate in insect cells.
• Compared with mammalian cells, the cell lines of insect culture are relatively easy to grow, and the suspension culture of insect cells can produce a large number of target proteins.

Vector Construction Services

Creative BioMart Vir-Sci has developed a range of proprietary technologies and reagents that have significantly improved the vector construction process and efficiency especially for the baculovirus/insect expression systems. Utilizing our vector production line, we can continuously provide high-quality customized products of vectors.

Service Advantages

• Provide vectors with high protein expression efficiency
• Help customers accelerate the research process
• Strict operation processes for vector construction services
• Competitive price in the market of vector construction services
• Ensure 24/7 online service

Workflow of Baculovirus Vector Construction at Creative BioMart Vir-Sci

Creative BioMart Vir-Sci has an expert team that own extensive experience in the field of vector construction, providing high quality of recombinant vector for baculovirus/insect expression systems, which can apply to preclinical cytological and holistic animal experiments. If you are seeking for related services, please feel free to contact us, our expert team will design high-quality vector construction solutions according to your needs and provide you with professional and thoughtful services.

References

1. Luckow V A.; et al. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. Journal of Virology, 1993, 67(8): 4566-4579.
2. Motohashi T.; et al. Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system. Biochemical and Biophysical Research Communications, 2005, 326(3): 564-569.