Viral Nonstructural Proteins Expression Service
Creative BioMart Vir-Sci has a mature viral nonstructural proteins expression platform. Our experts have extensive experience in the field of viral nonstructural proteins expression. Over the years, we have completed protein expression experiments for customers. These services have contributed to the clinical development process of many of our biopharmaceutical partners, including but not limited to codon optimization, protein expression analysis and identification, optimization of protein expression conditions. We have a complete protein expression research and development platform, and medical research provides a full range of services.
Non-Structural Protein
Non-structural protein refers to a protein encoded by the viral genome that has a certain function in the process of virus replication or gene expression regulation, but ultimately does not combine to produce a mature virus and is not a part of the virus structure. They usually include various enzymes and transcription factors used by the virus to replicate itself, such as viral proteases, RNA replicase or other template-oriented polymerases, and some means to control the host.
Our Services
- Replicon formation-related proteins expression service
- Immunomodulation-related proteins expression service
Examples of Viral Nonstructural Proteins
NSP1
NS1
NSP1 is a non-structural RNA-binding protein that contains cysteine-rich regions and is part of the early replication intermediates. The cysteine-rich region of NSP1 is not considered necessary for the rearrangement of heterologous viral genome segments.
Influenza non-structural protein (NS1) is a homodimeric RNA binding protein found in influenza viruses and is necessary for virus replication. NS1 binds to the polyA tails of mRNAs, keeping them in the nucleus. NS1 inhibits mRNA splicing by tightly binding to specific styloid processes of U6 snRNA.
Viral Nonstructural Proteins Expression Service Process
- Codon optimization. Creative BioMart Vir-Sci’s codon optimization technology involves gene synthesis, vector construction, gene assurance transcription, mRNA translation, post-translational modification and other links.
- Construction of recombinant expression plasmid, subcloning of the target gene and electrotransformation of recombinant plasmid into expression strains.
- Identification and screening of high expression strains.
- Protein expression analysis and identification.
- Optimization of protein expression conditions.
- Large-scale protein expression and purification.
- Protein identification.
- Use qualitative or quantitative analysis methods for protein identification.
Why Choose Creative BioMart Vir-Sci?
High success rate. Self-built laboratory, independent research and development of a number of experimental technologies, the experimental success rate is greater than 98%.
High-purity protein. Four major protein purification methods, combined with the protein purification system, can customize high-purity protein (>95%) for use in enzyme preparations, crystal structure analysis, biological activity experiments, etc.
One-stop service. only need to provide gene sequence, Creative BioMart Vir-Sci completes vector construction, protein expression, protein purification, antibody preparation, interaction analysis and other one-stop services.
Workflow of Viral Nonstructural Proteins Expression Service at Creative BioMart Vir-Sci
If you are looking for related services, please feel free to contact us. The experts of Creative BioMart Vir-Sci will communicate with you in time.
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